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Promega pgl-2 basic plasmid
Pgl 2 Basic Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl-2+basic+plasmid/pgl3+basic/pmc07545198-296-15-16
Average 90 stars, based on 1 article reviews
pgl-2 basic plasmid - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Plasmid Preparation:

Article Title: Direct Response Elements of BMP within the PV.1A Promoter Are Essential for Its Transcriptional Regulation during Early Xenopus Development
Article Snippet: A 3.8 kb fragment from a positive clone containing 2.5 kb of 5′-flanking region was subcloned into the pGL-2 basic plasmid (Promega, Madison, WI); the clone was designated the -2525 construct.

Article Title: The Rel family member P50 mediates cytokine-induced C-reactive protein expression by a novel mechanism.
Article Snippet: PGL-2 basic plasmid, luciferase assay kits, and purified recombinant human p50 (rhp50) protein were purchased from Promega (Madison, WI).

Article Title: An SCL 3' enhancer targets developing endothelium together with embryonic and adult haematopoietic progenitors.
Article Snippet: Blood formation begins early during embryogenesis in vertebrates and continues throughout the life of the animal to generate multiple lineages of mature cells.. Haematopoiesis occurs in both extra-embryonic and intra-embryonic sites during mouse and avian development (Dieterlen-Lievre, 1975; Dzierzak and Medvinsky, 1995; Godin et al., 1995, 1993; Medvinsky et al., 1993).. Murine multipotent progenitors and multipotential repopulating cells are also detected for the first time during development at day 8-9 of gestation in the yolk sac as well as within the embryo proper in the para-aortic splachnopleura (Cumano et al., 1996; Yoder et al., 1997).

Article Title: Foxd4l1.1 negatively regulates transcription of neural repressor ventx1.1 during neuroectoderm formation in Xenopus embryos
Article Snippet: The 2.5 kbs of 5′-flanking region of positive clone was subcloned into the pGL-2 basic plasmid (Promega, Madison, WI) and was designated as the − 2481 bps construct.

Article Title: Transcriptional regulation of insulin-like growth factor-II gene expression by cortisol in fetal sheep during late gestation.
Article Snippet: The resultant fragments were ligated into the HindIII site of pGL-2 basic plasmid (Promega, Southampton, UK), and designated pGL-2-P3 and pGL-2-P4, respectively.

Article Title: Ventx1.1 competes with a transcriptional activator Xcad2 to regulate negatively its own expression
Article Snippet: A 3.8-kb DNA fragment from the library screening, which contained 2.5 kb of 5′-flanking region of Ventx1.1 , was subcloned into pGL-2 basic plasmid (Promega) to produce a −2525-bp promoter construct.

Construct:

Article Title: Direct Response Elements of BMP within the PV.1A Promoter Are Essential for Its Transcriptional Regulation during Early Xenopus Development
Article Snippet: A 3.8 kb fragment from a positive clone containing 2.5 kb of 5′-flanking region was subcloned into the pGL-2 basic plasmid (Promega, Madison, WI); the clone was designated the -2525 construct.

Article Title: The Rel family member P50 mediates cytokine-induced C-reactive protein expression by a novel mechanism.
Article Snippet: PGL-2 basic plasmid, luciferase assay kits, and purified recombinant human p50 (rhp50) protein were purchased from Promega (Madison, WI).

Article Title: An SCL 3' enhancer targets developing endothelium together with embryonic and adult haematopoietic progenitors.
Article Snippet: Blood formation begins early during embryogenesis in vertebrates and continues throughout the life of the animal to generate multiple lineages of mature cells.. Haematopoiesis occurs in both extra-embryonic and intra-embryonic sites during mouse and avian development (Dieterlen-Lievre, 1975; Dzierzak and Medvinsky, 1995; Godin et al., 1995, 1993; Medvinsky et al., 1993).. Murine multipotent progenitors and multipotential repopulating cells are also detected for the first time during development at day 8-9 of gestation in the yolk sac as well as within the embryo proper in the para-aortic splachnopleura (Cumano et al., 1996; Yoder et al., 1997).

Article Title: Foxd4l1.1 negatively regulates transcription of neural repressor ventx1.1 during neuroectoderm formation in Xenopus embryos
Article Snippet: The 2.5 kbs of 5′-flanking region of positive clone was subcloned into the pGL-2 basic plasmid (Promega, Madison, WI) and was designated as the − 2481 bps construct.

Article Title: Transcriptional regulation of insulin-like growth factor-II gene expression by cortisol in fetal sheep during late gestation.
Article Snippet: The resultant fragments were ligated into the HindIII site of pGL-2 basic plasmid (Promega, Southampton, UK), and designated pGL-2-P3 and pGL-2-P4, respectively.

Article Title: Ventx1.1 competes with a transcriptional activator Xcad2 to regulate negatively its own expression
Article Snippet: A 3.8-kb DNA fragment from the library screening, which contained 2.5 kb of 5′-flanking region of Ventx1.1 , was subcloned into pGL-2 basic plasmid (Promega) to produce a −2525-bp promoter construct.

Library Screening:

Article Title: Direct Response Elements of BMP within the PV.1A Promoter Are Essential for Its Transcriptional Regulation during Early Xenopus Development
Article Snippet: A 3.8 kb fragment from a positive clone containing 2.5 kb of 5′-flanking region was subcloned into the pGL-2 basic plasmid (Promega, Madison, WI); the clone was designated the -2525 construct.

Article Title: The Rel family member P50 mediates cytokine-induced C-reactive protein expression by a novel mechanism.
Article Snippet: PGL-2 basic plasmid, luciferase assay kits, and purified recombinant human p50 (rhp50) protein were purchased from Promega (Madison, WI).

Article Title: An SCL 3' enhancer targets developing endothelium together with embryonic and adult haematopoietic progenitors.
Article Snippet: Blood formation begins early during embryogenesis in vertebrates and continues throughout the life of the animal to generate multiple lineages of mature cells.. Haematopoiesis occurs in both extra-embryonic and intra-embryonic sites during mouse and avian development (Dieterlen-Lievre, 1975; Dzierzak and Medvinsky, 1995; Godin et al., 1995, 1993; Medvinsky et al., 1993).. Murine multipotent progenitors and multipotential repopulating cells are also detected for the first time during development at day 8-9 of gestation in the yolk sac as well as within the embryo proper in the para-aortic splachnopleura (Cumano et al., 1996; Yoder et al., 1997).

Article Title: Foxd4l1.1 negatively regulates transcription of neural repressor ventx1.1 during neuroectoderm formation in Xenopus embryos
Article Snippet: The 2.5 kbs of 5′-flanking region of positive clone was subcloned into the pGL-2 basic plasmid (Promega, Madison, WI) and was designated as the − 2481 bps construct.

Article Title: Transcriptional regulation of insulin-like growth factor-II gene expression by cortisol in fetal sheep during late gestation.
Article Snippet: The resultant fragments were ligated into the HindIII site of pGL-2 basic plasmid (Promega, Southampton, UK), and designated pGL-2-P3 and pGL-2-P4, respectively.

Article Title: Ventx1.1 competes with a transcriptional activator Xcad2 to regulate negatively its own expression
Article Snippet: A 3.8-kb DNA fragment from the library screening, which contained 2.5 kb of 5′-flanking region of Ventx1.1 , was subcloned into pGL-2 basic plasmid (Promega) to produce a −2525-bp promoter construct.

Luciferase:

Article Title: Direct Response Elements of BMP within the PV.1A Promoter Are Essential for Its Transcriptional Regulation during Early Xenopus Development
Article Snippet: A 3.8 kb fragment from a positive clone containing 2.5 kb of 5′-flanking region was subcloned into the pGL-2 basic plasmid (Promega, Madison, WI); the clone was designated the -2525 construct.

Article Title: The Rel family member P50 mediates cytokine-induced C-reactive protein expression by a novel mechanism.
Article Snippet: PGL-2 basic plasmid, luciferase assay kits, and purified recombinant human p50 (rhp50) protein were purchased from Promega (Madison, WI).

Article Title: An SCL 3' enhancer targets developing endothelium together with embryonic and adult haematopoietic progenitors.
Article Snippet: Blood formation begins early during embryogenesis in vertebrates and continues throughout the life of the animal to generate multiple lineages of mature cells.. Haematopoiesis occurs in both extra-embryonic and intra-embryonic sites during mouse and avian development (Dieterlen-Lievre, 1975; Dzierzak and Medvinsky, 1995; Godin et al., 1995, 1993; Medvinsky et al., 1993).. Murine multipotent progenitors and multipotential repopulating cells are also detected for the first time during development at day 8-9 of gestation in the yolk sac as well as within the embryo proper in the para-aortic splachnopleura (Cumano et al., 1996; Yoder et al., 1997).

Article Title: Foxd4l1.1 negatively regulates transcription of neural repressor ventx1.1 during neuroectoderm formation in Xenopus embryos
Article Snippet: The 2.5 kbs of 5′-flanking region of positive clone was subcloned into the pGL-2 basic plasmid (Promega, Madison, WI) and was designated as the − 2481 bps construct.

Article Title: Transcriptional regulation of insulin-like growth factor-II gene expression by cortisol in fetal sheep during late gestation.
Article Snippet: The resultant fragments were ligated into the HindIII site of pGL-2 basic plasmid (Promega, Southampton, UK), and designated pGL-2-P3 and pGL-2-P4, respectively.

Article Title: Ventx1.1 competes with a transcriptional activator Xcad2 to regulate negatively its own expression
Article Snippet: A 3.8-kb DNA fragment from the library screening, which contained 2.5 kb of 5′-flanking region of Ventx1.1 , was subcloned into pGL-2 basic plasmid (Promega) to produce a −2525-bp promoter construct.

Purification:

Article Title: Direct Response Elements of BMP within the PV.1A Promoter Are Essential for Its Transcriptional Regulation during Early Xenopus Development
Article Snippet: A 3.8 kb fragment from a positive clone containing 2.5 kb of 5′-flanking region was subcloned into the pGL-2 basic plasmid (Promega, Madison, WI); the clone was designated the -2525 construct.

Article Title: The Rel family member P50 mediates cytokine-induced C-reactive protein expression by a novel mechanism.
Article Snippet: PGL-2 basic plasmid, luciferase assay kits, and purified recombinant human p50 (rhp50) protein were purchased from Promega (Madison, WI).

Article Title: An SCL 3' enhancer targets developing endothelium together with embryonic and adult haematopoietic progenitors.
Article Snippet: Blood formation begins early during embryogenesis in vertebrates and continues throughout the life of the animal to generate multiple lineages of mature cells.. Haematopoiesis occurs in both extra-embryonic and intra-embryonic sites during mouse and avian development (Dieterlen-Lievre, 1975; Dzierzak and Medvinsky, 1995; Godin et al., 1995, 1993; Medvinsky et al., 1993).. Murine multipotent progenitors and multipotential repopulating cells are also detected for the first time during development at day 8-9 of gestation in the yolk sac as well as within the embryo proper in the para-aortic splachnopleura (Cumano et al., 1996; Yoder et al., 1997).

Article Title: Foxd4l1.1 negatively regulates transcription of neural repressor ventx1.1 during neuroectoderm formation in Xenopus embryos
Article Snippet: The 2.5 kbs of 5′-flanking region of positive clone was subcloned into the pGL-2 basic plasmid (Promega, Madison, WI) and was designated as the − 2481 bps construct.

Article Title: Transcriptional regulation of insulin-like growth factor-II gene expression by cortisol in fetal sheep during late gestation.
Article Snippet: The resultant fragments were ligated into the HindIII site of pGL-2 basic plasmid (Promega, Southampton, UK), and designated pGL-2-P3 and pGL-2-P4, respectively.

Article Title: Ventx1.1 competes with a transcriptional activator Xcad2 to regulate negatively its own expression
Article Snippet: A 3.8-kb DNA fragment from the library screening, which contained 2.5 kb of 5′-flanking region of Ventx1.1 , was subcloned into pGL-2 basic plasmid (Promega) to produce a −2525-bp promoter construct.

Recombinant:

Article Title: Direct Response Elements of BMP within the PV.1A Promoter Are Essential for Its Transcriptional Regulation during Early Xenopus Development
Article Snippet: A 3.8 kb fragment from a positive clone containing 2.5 kb of 5′-flanking region was subcloned into the pGL-2 basic plasmid (Promega, Madison, WI); the clone was designated the -2525 construct.

Article Title: The Rel family member P50 mediates cytokine-induced C-reactive protein expression by a novel mechanism.
Article Snippet: PGL-2 basic plasmid, luciferase assay kits, and purified recombinant human p50 (rhp50) protein were purchased from Promega (Madison, WI).

Article Title: An SCL 3' enhancer targets developing endothelium together with embryonic and adult haematopoietic progenitors.
Article Snippet: Blood formation begins early during embryogenesis in vertebrates and continues throughout the life of the animal to generate multiple lineages of mature cells.. Haematopoiesis occurs in both extra-embryonic and intra-embryonic sites during mouse and avian development (Dieterlen-Lievre, 1975; Dzierzak and Medvinsky, 1995; Godin et al., 1995, 1993; Medvinsky et al., 1993).. Murine multipotent progenitors and multipotential repopulating cells are also detected for the first time during development at day 8-9 of gestation in the yolk sac as well as within the embryo proper in the para-aortic splachnopleura (Cumano et al., 1996; Yoder et al., 1997).

Article Title: Foxd4l1.1 negatively regulates transcription of neural repressor ventx1.1 during neuroectoderm formation in Xenopus embryos
Article Snippet: The 2.5 kbs of 5′-flanking region of positive clone was subcloned into the pGL-2 basic plasmid (Promega, Madison, WI) and was designated as the − 2481 bps construct.

Article Title: Transcriptional regulation of insulin-like growth factor-II gene expression by cortisol in fetal sheep during late gestation.
Article Snippet: The resultant fragments were ligated into the HindIII site of pGL-2 basic plasmid (Promega, Southampton, UK), and designated pGL-2-P3 and pGL-2-P4, respectively.

Article Title: Ventx1.1 competes with a transcriptional activator Xcad2 to regulate negatively its own expression
Article Snippet: A 3.8-kb DNA fragment from the library screening, which contained 2.5 kb of 5′-flanking region of Ventx1.1 , was subcloned into pGL-2 basic plasmid (Promega) to produce a −2525-bp promoter construct.



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